Table 1.

Cell Size, Cell Number, and Number of Stomata in the First Leaves of Wild-Type, cycd4 Mutant, and CYCD4-OE Seedlings

Line	Leaf Blade Area (mm2)	Cell Area (μm2)	Cell Number	Number of Stomata	Stomatal Index (%)
Columbia wild type	40.02 ± 2.58	1878 ± 98	21487 ± 1104	4125 ± 314	19.12 ± 0.84
cycd4;1-2	35.41 ± 2.11	1865 ± 89	19224 ± 1240	3551 ± 241	18.38 ± 0.44
cycd4;2-2	36.95 ± 1.34	1959 ± 47	18918 ± 745	3380 ± 214	17.82 ± 0.77
cycd4;2-3	37.62 ± 1.61	2094 ± 116	18292 ± 795*	3424 ± 229	18.59 ± 0.75
cycd4;1-2 cycd4;2-2	37.44 ± 1.65	1789 ± 47	21244 ± 1381	3835 ± 264	18.09 ± 0.41
cycd4;1-2 cycd4;2-3	36.96 ± 2.48	1732 ± 58	21639 ± 1646	3981 ± 367	18.19 ± 0.41
35S:CYCD4;1 (F12)	40.55 ± 2.86	1295 ± 63***	31662 ± 2223***	5206 ± 317*	16.64 ± 0.54*
35S:CYCD4;2 (O3)	40.14 ± 2.72	1554 ± 48**	28785 ± 1838**	4276 ± 350	16.27 ± 0.38**

The cell size, cell number, and number of stomata were estimated for the abaxial epidermal cells. All measurements were performed using seedlings at 20 d after germination. Data are presented as mean ± se (n = 12). Significant differences between the wild-type and mutant or transgenic plants are as follows: *P < 0.05, **P < 0.01, and ***P < 0.001; the other values are not significant (P > 0.05).