Figure 2.

Response of Arabidopsis cdkc;2 and cyct1;5 Mutants to TMV-cg and CaLCuV.

(A) Accumulation of TMV-cg viral RNAs. Three leaves of 4-week-old Arabidopsis plants were inoculated with TMV-cg (5 μg/mL). The lower, inoculated leaves and upper, systemic infected leaves of five plants were collected at the indicated DAI for RNA isolation. RNA gel blot analysis was performed with ³²P-labeled TMV-cg coat protein gene as a probe.

(B) Accumulation of CaLCuV DNA-A and DNA-B. Four-week-old Arabidopsis plants were bombarded with the infectious DNA clones of CaLCuV. Total DNA was isolated from infected leaves at the indicated DAI. DNA gel blot analysis was performed with ³²P-labeled DNA fragments specific to the A or B components of CaLCuV.