Figure 3.

CDKC;2 and CYCT1;5 Dependence of the CaMV 35S Promoter Activity.

(A) GUS activities in the wild type, cdkc;2, and cyct1;5 mutants transformed with a GUS transgene driven by the CaMV 35S promoter. Average GUS activities were calculated from 15 to 20 independent T1 transformants. GUS activities are expressed in units (nanomoles of 4-methylumbelliferone per minute per milligram of total soluble protein).

(B) Accumulation of GUS transcripts. Total RNA was pooled from 15 to 20 T1 transformants and probed with the full-length GUS gene fragment.