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. 2007 Apr;19(4):1388–1402. doi: 10.1105/tpc.107.051375

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Copyright © 2007, American Society of Plant Biologists

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Figure 4. — CaMV Resistance of the cdkc;2/CDKC;1 RNAi and cyct1;5/CYCT1;4 RNAi Mutants.

(A) Viral DNA accumulation. Wild-type (lane 1), cdkc;2 cyct1;5 (lane 2), cdkc;2/CDKC;1 RNAi (lane 3), and cyct1;5/CYCT1;4 RNAi (lane 4) plants were inoculated by bombardment of pCa122, and total DNA was isolated from leaf tissues harvested at the indicated DAI and probed with the P6 open reading frame of CaMV. Ethidium bromide staining of genomic DNA is shown as a loading control.

(B) Viral RNA accumulation. Total RNA was isolated from inoculated wild-type (lane 1), cdkc;2 cyct1;5 (lane 2), cdkc;2/CDKC;1 RNAi (lane 3), and cyct1;5/CYCT1;4 RNAi (lane 4) plants. The RNA gel blot was first probed with the P3 open reading frame of CaMV for detection of the 35S RNA. The blot was stripped and reprobed with the P6 open reading frame of CaMV for detection of the 19S RNA. Ethidium bromide staining of rRNA is shown for the assessment of equal loading.