Figure 1.

Immunolocalization and Membrane Association of DEG5 and DEG8.

(A) and (B) Intact thylakoid membranes (A) and sonicated thylakoid membranes (B) from mature Arabidopsis leaves were incubated with trypsin for 15 min at 25°C.

(C) and (D) The sonicated thylakoid membranes were incubated with 1 M NaCl (C) or 1 M CaCl₂ (D) for 30 min at 4°C. After these treatments, the thylakoid proteins were separated by SDS-PAGE and immunodetected with anti-DEG5, anti-DEG8, anti-PsbO, and anti-CP47 antibodies.

PsbO and CP47 were used as markers. Membrane preparations that had not been subjected to any of these treatments were used as controls. The experiments were repeated five times independently, and similar results were obtained. Results of a representative experiment are shown.