Figure 12.

Immunodetection of the Degradation Products of the D1 Protein in the Thylakoid Membranes Isolated from High Light–Treated Arabidopsis Leaves.

Thylakoid membranes were isolated from wild-type (A) and deg5 deg8 (B) leaves that had been exposed to high-light illumination for 1 and 2 h. The thylakoid membrane proteins were separated by SDS-PAGE and immunodetected with antibodies raised against the N-terminal residues (58 to 86) of the D1 protein. Control indicates samples containing recombinant DEG8 and high light–treated thylakoid membranes. The degradation fragment of the D1 protein is indicated with an arrow. Similar results were obtained in two additional independent experiments.