Figure 2.
Quantitative suberin composition in isolated soybean root tissues. Epidermal and endodermal tissues were isolated from soybean roots separated into two developmental stages, state I (0–70 mm from the root tip) and state II (90–160 mm from the root tip), by exhaustive enzymatic digestion and manual dissection. Isolated tissues were subjected to BF3/MeOH transesterification to release (A) poly(aliphatic) and (B) esterified phenolic components or alkaline NBO to release (C) poly(phenolic) components. D, Suberin-associated waxes were isolated by solvent extraction. White bars, ‘Conrad.’ Black bars, OX760-6. See “Materials and Methods” for details. Data are expressed on the basis of root or endodermal surface area. Bars represent the average ± se of six measurements; that is, triplicate measurements in each of two independent replicates. Bars labeled with the same letter were not significantly different (lsd = 0.05).