Table II.

Chain length distribution of soybean root suberin-associated waxes by substance class

Suberin-associated waxes were solvent extracted from state I (0–70 mm from root tip) and state II (90–160 mm from root tip) epidermal and endodermal tissues of two soybean genotypes and subjected to GC analysis (see “Materials and Methods”). Values (μg cm⁻² ± se) are the average of two replicate experiments (with three analyses per experiment).

Substance Class	Chain Length	Epidermis				Endodermis
		‘Conrad’		OX760-6		‘Conrad’		OX760-6
		0–70 mm	90–160 mm	0–70 mm	90–160 mm	0–70 mm	90–160 mm	0–70 mm	90–160 mm
Fatty acids	16:0	0.02 ± 0.001	0.01 ± 0.001	0.02 ± 0.005	0.02 ± 0.004	0.10 ± 0.01	0.10 ± 0.001	0.16 ± 0.07	0.12 ± 0.08
	17:0	0.01 ± 0.008	0.01 ± 0.001	0.01 ± 0.001	0.01 ± 0.002	0.07 ± 0.008	0.10 ± 0.008	0.03 ± 0.002	0.06 ± 0.002
	18:2	0.01 ± 0.002	0.01 ± 0.004	0.01 ± 0.001	0.01 ± 0.003	0.05 ± 0.001	0.10 ± 0.006	0.02 ± 0.001	0.05 ± 0.003
	18:1	0.01 ± 0.001	0.01 ± 0.001	0.01 ± 0.001	0.01 ± 0.001	0.05 ± 0.001	0.10 ± 0.001	0.03 ± 0.004	0.04 ± 0.002
	18:0	0.01 ± 0.005	0.01 ± 0.002	0.01 ± 0.001	0.04 ± 0.006	0.18 ± 0.03	0.23 ± 0.001	0.08 ± 0.003	0.13 ± 0.09
	20:0	0.01 ± 0.001	0.01 ± 0.002	0.01 ± 0.001	0.01 ± 0.001	0.07 ± 0.002	0.11 ± 0.001	0.04 ± 0.003	0.06 ± 0.003
	21:0	0.01 ± 0.008	0.01 ± 0.004	0.01 ± 0.001	0.01 ± 0.001	0.05 ± 0.001	0.13 ± 0.003	0.03 ± 0.001	0.06 ± 0.006
	22:0	0.01 ± 0.009	0.01 ± 0.001	0.01 ± 0.001	0.01 ± 0.003	0.07 ± 0.003	0.12 ± 0.001	0.04 ± 0.003	0.07 ± 0.004
	23:0	0.01 ± 0.005	0.01 ± 0.002	0.01 ± 0.001	0.01 ± 0.003	0.08 ± 0.001	0.11 ± 0.001	0.01 ± 0.001	0.07 ± 0.002
	24:0	0.01 ± 0.001	0.02 ± 0.004	0.01 ± 0.001	0.01 ± 0.002	0.07 ± 0.005	0.12 ± 0.001	0.05 ± 0.007	0.08 ± 0.002
	25:0	0.01 ± 0.007	0.01 ± 0.007	0.01 ± 0.001	0.01 ± 0.004	0.09 ± 0.01	0.11 ± 0.003	0.04 ± 0.001	0.08 ± 0.02
	Total	0.12 ± 0.003	0.12 ± 0.001	0.12 ± 0.01	0.15 ± 0.01	0.88 ± 0.03	1.33 ± 0.03	0.53 ± 0.11	0.82 ± 0.05
1-Alkanols	24:0	0.01 ± 0.001	0.01 ± 0.003	0.01 ± 0.001	0.01 ± 0.001	0.04 ± 0.001	0.04 ± 0.001	0.04 ± 0.01	0.03 ± 0.001
	25:0	0.01 ± 0.001	0.01 ± 0.001	0.01 ± 0.001	0.01 ± 0.001	0.03 ± 0.005	0.02 ± 0.001	0.02 ± 0.001	0.04 ± 0.001
	26:0	0.01 ± 0.001	0.01 ± 0.001	0.01 ± 0.001	0.01 ± 0.001	0.03 ± 0.001	0.08 ± 0.001	0.01 ± 0.005	0.02 ± 0.001
	Total	0.03 ± 0.001	0.03 ± 0.001	0.03 ± 0.003	0.03 ± 0.003	0.10 ± 0.002	0.14 ± 0.004	0.07 ± 0.01	0.09 ± 0.003
Alkanes	23:0	0.02 ± 0.003	0.04 ± 0.002	0.02 ± 0.006	0.02 ± 0.001	0.25 ± 0.008	0.31 ± 0.001	0.11 ± 0.006	0.22 ± 0.02
	24:0	0.02 ± 0.001	0.04 ± 0.008	0.02 ± 0.006	0.03 ± 0.001	0.22 ± 0.01	0.32 ± 0.001	0.12 ± 0.001	0.22 ± 0.02
	25:0	0.04 ± 0.004	0.08 ± 0.006	0.04 ± 0.001	0.04 ± 0.001	0.35 ± 0.004	0.57 ± 0.001	0.21 ± 0.001	0.39 ± 0.002
	26:0	0.05 ± 0.005	0.10 ± 0.001	0.03 ± 0.01	0.04 ± 0.001	0.51 ± 0.002	0.68 ± 0.002	0.25 ± 0.001	0.46 ± 0.05
	27:0	0.18 ± 0.02	0.09 ± 0.001	0.05 ± 0.001	0.05 ± 0.001	0.43 ± 0.001	0.76 ± 0.002	0.52 ± 0.02	0.47 ± 0.05
	28:0	0.15 ± 0.008	0.23 ± 0.002	0.10 ± 0.03	0.13 ± 0.01	1.00 ± 0.004	0.65 ± 0.007	0.62 ± 0.001	1.15 ± 0.05
	29:0	0.11 ± 0.009	0.24 ± 0.008	0.11 ± 0.001	0.12 ± 0.002	1.11 ± 0.001	1.77 ± 0.01	0.64 ± 0.01	1.17 ± 0.006
	31:0	0.12 ± 0.001	0.16 ± 0.01	0.11 ± 0.01	0.10 ± 0.002	1.10 ± 0.07	1.79 ± 0.10	0.42 ± 0.02	0.85 ± 0.02
	32:0	0.10 ± 0.001	0.14 ± 0.008	0.08 ± 0.02	0.07 ± 0.004	0.90 ± 0.02	1.28 ± 0.14	0.42 ± 0.01	0.70 ± 0.09
	Total	0.79 ± 0.004	1.12 ± 0.02	0.56 ± 0.07	0.60 ± 0.008	5.87 ± 0.06	9.04 ± 0.12	3.31 ± 0.02	5.63 ± 0.09
Overall Total		0.94 ± 0.05	1.28 ± 0.02	0.71 ± 0.08	0.78 ± 0.01	6.85 ± 0.09	10.43 ± 0.03	3.91 ± 0.09	6.54 ± 0.02