Figure 1.

Effects of anoxia on rice coleoptile growth and ADH gene expression. A, Growth pattern of rice coleoptiles in air and anoxia; rice seeds were germinated in air and anoxia and the coleoptile length recorded daily. Data are means of 20 replicates ±sd. B, Pattern of expression of ADH1 (Os11g10480) and ADH2 (Os11g10510) genes in coleoptiles from rice seeds germinated in air and anoxia. Relative expression level (REL), measured by real-time reverse transcription (RT)-PCR, is shown as a heat map (REL, 1 = expression data from the aerobic coleoptile at day 2). Data are means of three replicates ±sd. C, Growth pattern of rice coleoptiles and roots from seedlings germinated under aerobic conditions for 4 d and then transferred to anoxia for an additional 48-h period; the coleoptile and root length were recorded during 48-h anoxic treatment as well as in air. Data are means of 20 replicates ±sd. D, Pattern of expression of ADH1 and ADH2 genes in the coleoptiles and roots from rice seeds germinated in air for 4 d and then transferred to anoxia for an additional 48-h period; REL, measured by real-time RT-PCR, is shown as a heat map (REL, 1 = expression data from the aerobic coleoptile at the beginning of the experiment, t = 0); data are means of three replicates (see Supplemental Table S3 for ±sd values). E, Pattern of expression of ADH1 and ADH2 genes in the leaves, internodes, and roots from rice plants grown in air for 20 d and then transferred to anoxia for an additional 3- to 6-h period; REL, measured by real-time RT-PCR, is shown as a heat map (REL, 1 = expression data from the aerobic leaf at the beginning of the experiment, t = 0); data are means of three replicates (see Supplemental Table S3 for ±sd values).