Figure 8.

Effects of anoxia on P450 gene expression. A, Pattern of expression of P450 genes differentially regulated by anoxia; data are expressed as a heat map showing the signal intensity from the microarray experiment (data are means of two GeneChip experiments) performed on the coleoptiles from rice seeds germinated in air and anoxia for 4 d. Fold change is shown on the right side of the heat map (FDR P value < 0.01). B, Pattern of expression of the Os11g18570 gene in the coleoptiles from rice seeds germinated in air and anoxia; relative expression level (REL), measured by real-time reverse transcription (RT)-PCR, is shown in the graph (REL, 1 = expression data from the aerobic coleoptile at day 2); data are means of three replicates ±sd. C, Pattern of expression of the Os11g18570 gene in coleoptiles and roots from rice seeds germinated in air for 4 d and then transferred to anoxia for an additional 48-h period; REL, measured by real-time RT-PCR, is shown as a heat map (REL, 1 = expression data from the aerobic coleoptile at the beginning of the experiment, t = 0); data are means of three replicates (see Supplemental Table S3 for ±sd values). D, Pattern of expression of the Os11g18570 gene in the leaves, internodes, and roots from the rice plants grown in air for 20 d and then transferred to anoxia for an additional 6-h period; REL, measured by real-time RT-PCR, is shown as a heat map (REL, 1 = expression data from the aerobic leaf at the beginning of the experiment, t = 0); data are means of three replicates (see Supplemental Table S3 for ±sd values).