Figure 5.

Steady-state mRNA transcript levels of MAM1 and MAM3 in tissues of Col-0 wild-type and MAM3 mutant lines determined by RT-PCR. DNA fragments for individual transcripts of ACT8 (actin), MAM1, and MAM3 were generated by PCR amplification of products from RT activity primed by oligo(dT) hybridization to the RNA. The PCR products are shown after separation on 1% agarose gels stained with ethidium bromide. Tissue source: 1, roots; 2, mature leaves; 3, expanding leaves; 4, flowers; and 5, siliques.