Table III.
Kinetic parameters for various 2-oxo acids with recombinant MAM3a
| Substrate | Km | Vmax | kcat | kcat/Km |
|---|---|---|---|---|
| μm | nmol min−1mg−1 | s−1 | m−1s−1 | |
| 4-Methylthio-2-oxobutanoic acid (→C3 glucosinolates) | 932 ± 56 | 1,448 ± 299 | 1.3 ± 0.3 | 1,380 |
| 5-Methylthio-2-oxopentanoic acid (→C4 glucosinolates) | 476 ± 199 | 1,495 ± 679 | 1.3 ± 0.6 | 2,730 |
| 6-Methylthio-2-oxohexanoic acid (→C5 glucosinolates) | 463 ± 210 | 2,869 ± 768 | 2.5 ± 0.7 | 5,400 |
| 8-Methylthio-2-oxooctanoic acid (→C7 glucosinolates) | 253 ± 123 | 364 ± 143 | 0.3 ± 0.1 | 1,280 |
| 9-Methylthio-2-oxononanoic acid (→C8 glucosinolates) | 81 ± 21 | 31 ± 8 | 0.03 ± 0.01 | 370 |
| 2-Oxoisovalerate | 1,000 ± 200 | 199 ± 38 | 0.18 ± 0.03 | 200 |
| Pyruvate | 8,600 ± 4,300 | 191 ± 48 | 0.17 ± 0.04 | 23 |
| Acetyl-CoA | 2,300 ± 1,200 | 3,344 ± 1428 | 3.0 ± 1.3 | 1,300 |
a
Data presented are means ± sds of at least five replicates per substrate.