Figure 5.

The synthesis of thylakoid membrane proteins in wild-type and photorespiratory pathway mutants in air. In A, leaf discs (78.5 mm²) were vacuum infiltrated with [³⁵S]Met/Cys (10 mCi ml⁻¹) solution and floated on water during exposure to light at 200 μmol photons m⁻² s⁻¹ for 30 min. Thylakoids were isolated from the leaf discs and thylakoid proteins (corresponding to 7.85 mm²) were separated by NuPAGE Novex 4%-12% Bis-Tris gel electrophoresis. In B, attached leaves were preincubated for 3 or 6 h in the light (200 μmol photons m⁻² s⁻¹) in air before leaf discs were isolated and infiltrated with [³⁵S]Met/Cys solution for incorporation into proteins as described in A.