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Acyl-CoA profiles of transgenic Arabidopsis expressing A. leveillei desaturase AL21. Acyl-CoA pools of Arabidopsis leaves from wild-type plants (A) and AL21 transgenic plants (B) were analyzed by HPLC, with detection of etheno-derivatives at 350 nm. The presence of additional peaks is indicated with stars. The internal standard is 17:0-acyl-CoA. It was not possible to determine if the 18:2, n-6 and 18:3, n-3 peaks contained additional (Δ5-NMI) products (e.g. 18:2Δ5,9; 18:3Δ5,9,12).
