Phylogenetic distribution of intron 24 and of the three novel introns, A, B, and C, detected in the Xdh studied region. Presence of an intron is represented by a plus sign, and absence is represented by a minus. The putative phylogenetic insertion of the novel introns is indicated by arrows. The consensus cladogram is based on data from refs. 22–24. The number of species included within taxonomic categories is shown in parentheses. The species are: for the willistoni subgroup, D. equinoxialis, D. paulistorum, D. willistoni, D. tropicalis, and D. insularis; for the saltans group, D. saltans, D. prosaltans, D. emarginata, D. neocordata, D. sturtevanti, and D. subsaltans; for the melanogaster group, D. melanogaster, D. simulans, D. teissieri, and D. erecta; for the obscura group, D. subobscura, D. pseudoobscura, and D. persimilis; for the Drosophila subgenus, D. gymnobasis, D. robusta, D. virilis, D. hydei, D. funebris, and D. busckii; Zaprionus tuberculatus; for Hirtodrosophila, D. pictiventris; Scaptodrosophila lebanonensis; Chymomyza amoena; and Ceratitis capitata. The nucleotide sequences of the introns in the Drosophilid species have GenBank accession numbers AF039603–AF039649.