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Three alleles of T-DNA insertional chx20 mutants. A, Wild-type (WT) and mutant plants look similar. Sites of T-DNA insertion for chx20-1, chx20-3, and chx20-4 mutants are shown in Figure 1A. B, Mutants lack CHX20 transcript. RNA isolated from leaves of chx20-1, chx20-3, and chx20-4 and wild type was reverse transcribed. The cDNA product was PCR amplified with primers F1 and R1, F2 and R2, and F3 and R2 shown in the genomic structure (top). Actin11 is amplified as a loading control.
