Figure 5.

Robust markers for MDE development. The number listed beside each marker corresponds to the assigned probe number. The expression profile of a representative probe is presented when a marker is represented by multiple probes (number of multiple probes indicated in parentheses). The expression ratio (log₂ scale) of each probe or probe set relative to the common reference is indicated. Color coding of the expression ratios is as in Supplemental Figure S4. S, Probes that are expressed at significantly higher levels in preglobular suspensor-bearing MDEs as compared to preglobular conventional MDEs. 0d, Starting microspore culture; 5p, 5-d pollen culture; 2e, 2-d conventional MDE culture; 5e, 5-d conventional MDE culture; 10e, 10-d conventional MDE culture; 8se, 8-d suspensor-bearing MDE culture; 10se, 10-d suspensor-bearing MDE culture; LF, mature leaves; FB, flower buds (3–4 mm).