Table I.
Composition of rapeseed MDE cultures used for the transcriptome analysis
Values are expressed as percentages of structures showing pollen or embryo development at the time of harvest relative to the number of microspores present at the start of culture. The 10-d embryo cultures were sieved through nylon filters to obtain pure populations of embryos. Note that the total embryo yield generally decreases as the MDE cultures age. 0d, Freshly isolated microspores; 5p, 5-d pollen culture; 2e, 2-d conventional MDE culture; 5e, 5-d conventional MDE culture; 10e, 10-d conventional MDE culture; 8se, 8-d suspensor-bearing MDE culture; 10se, 10-d suspensor-bearing MDE culture.
| Culture | Culture 1
|
Culture 2
|
||
|---|---|---|---|---|
| % Embryo | % Pollen | % Embryo | % Pollen | |
| 0d | – | – | – | – |
| 5p | 0 | 75 | 0 | 75 |
| 2e | 14 | 27 | 12 | 40 |
| 5e | 17 | 5 | 11 | 13 |
| 10e | 7 | 0 | 5 | 0 |
| 8se | 17 | 0 | 12 | 0 |
| 10se | 14 | 0 | 14 | 0 |