Table III.
Differentially expressed proteins identified in rapeseed MDE cultures
Each entry contains a number that corresponds to the protein spots indicated in Figure 4. Matched Peptides gives the number of peptide sequences identified by LC-MS/MS that showed homology to the best-matching gene product. The molecular mass and isoelectric point (IEP) as estimated from the 2-D gel are given, and in parentheses the deduced molecular mass and IEP of the best-matching gene product. The last column represents the percentage of sequence coverage by the matched peptides to the most similar protein in the database. At, Arabidopsis; Bj, Brassica juncea; Bn, rapeseed; Bo, Brassica oleracea; Br, Brassica rapa.
| No. | Best-Matching Gene Product (Organism) | ID | Process | Matched Peptides (n) | Molecular Mass Hit (kD) | IEP Hit (pI) | Coverage (%) |
|---|---|---|---|---|---|---|---|
| 1 | Expressed protein (At) | At5g48480 | Unknown | 3 | 20.1 (17.6) | 4.60 (4.80) | 16.8 |
| 2 | Proliferating cellular nuclear antigen (At) | At2g29570 | DNA replication | 5 | 35.7 (29.2) | 4.49 (4.39) | 36.7 |
| Proliferating cellular nuclear antigen (Bn) | gi15222379 | DNA replication | 5 | 35.7 (29.2) | 4.49 (4.42) | 36.7 | |
| 3 | 14-3-3 protein (Bn) | gi13447112 | Signaling | 5 | 33.5 (18.2) | 4.64 (5.19) | 20.8 |
| 4 | Disulfide isomerase-like protein (At) | At1g21750 | Redox processes | 14 | 61.3 (55.6) | 4.66 (4.61) | 25.9 |
| 5 | CCAAT-box binding factor Hap5a subunit (At) | At1g08970 | Transcription | 4 | 31.2 (25.6) | 5.02 (4.91) | 16.4 |
| 6 | Rubisco subunit binding protein (Bn) | gi464727 | Protein modification/processing | 8 | 62.7 (61.6) | 4.81 (4.95) | 18.7 |
| 7 | P-ribosylformylglycinamidine synthase (At) | At1g74260 | Secondary metabolism | 2 | 126.1 (151.7) | 4.97 (4.99) | 2.1 |
| 8 | Thiazole biosynthetic enzyme precursor (At) | At5g54770 | Secondary metabolism | 3 | 34.6 (36.6) | 5.06 (5.78) | 9.7 |
| 9 | Monodehydroascorbate reductase (At) | At5g03630 | Redox processes | 2 | 49.3 (47.5) | 5.11 (5.06) | 6.7 |
| 10a | 40S ribosomal protein (Bn) | gi15214300 | Protein synthesis | 3 | 42.3 (32.0) | 5.12 (4.94) | 13.7 |
| 10b | Gln synthetase (Bn) | gi1084350 | Amino acid metabolism | 3 | 42.3 (39.1) | 5.12 (5.13) | 9.5 |
| 11 | H+-transporting two-sector ATPase (At) | At3g28710 | Electron transport | 3 | 42.4 (40.7) | 5.16 (4.81) | 12.8 |
| 12 | HSP90 heat shock protein (At) | At2g04030 | Protein modification/processing | 2 | 97.7 (88.6) | 5.17 (4.74) | 3.5 |
| 13 | GST (At) | gi15218518 | Redox processes | 2 | 26.5 (18.8) | 5.39 (5.64) | 13.5 |
| 14 | Chloroplast Cpn21 protein (At) | At5g20720 | Protein modification/processing | 5 | 28.7 (26.8) | 5.46 (9.35) | 21.3 |
| 15 | Lactoylglutathione lyase/glyoxylase I (Bo) | gi2494843 | Redox processes | 11 | 34.2 (31.6) | 5.31 (4.97) | 24.8 |
| 16 | Dehydroascorbate reductase (Bj) | gi22653413 | Redox processes | 3 | 30.7 (28.5) | 5.47 (8.40) | 11.7 |
| Dehydroascorbate reductase (Bj) | gi22653415 | Redox processes | 3 | 30.7 (24.3) | 5.47 (5.19) | 13.8 | |
| 17 | Phoshoglycerate kinase (At) | At1g56190 | Carbohydrate metabolism | 7 | 44.2 (49.9) | 5.32 (6.23) | 22.4 |
| 18 | Peroxiredoxin (Br) | gi4928472 | Redox processes | 6 | 21.5 (17.4) | 5.48 (5.23) | 41.3 |
| 19 | Expressed protein (At) | At5g02240 | Unknown | 10 | 33.9 (27.1) | 5.57 (6.20) | 19.3 |
| 20 | Phosphoglycerate kinase (At) | At1g79550 | Carbohydrate metabolism | 10 | 42.5 (42.1) | 5.48 (5.33) | 23.2 |
| 21 | Chaperonin (At) | At3g13860 | Protein modification/processing | 13 | 60.5 (60.4) | 5.54 (5.74) | 13.8 |
| 22 | Chaperonin; T-complex protein (At) | At3g03960 | Protein modification/processing | 2 | 60.8 (58.9) | 5.63 (5.07) | 2.7 |
| 23 | Dehydroascorbate reductase (Br) | gi33285914 | Redox processes | 4 | 29.6 (12.0) | 5.86 (6.16) | 33.3 |
| 24 | Expressed protein (At) | At5g02240 | Unknown | 6 | 32.9 (27.1) | 5.87 (6.20) | 6.3 |
| 25 | l-Ascorbate peroxidase (Bn) | gi1890354 | Redox processes | 13 | 30.9 (27.7) | 5.96 (5.83) | 42.4 |
| 26 | Glutamate decarboxylase (At) | At2g02010 | Signaling | 4 | 57.3 (55.9) | 6.09 (6.26) | 6.4 |
| 27a | Lectin (At) | At3g16400 | Carbohydrate binding | 2 | 65.5 (51.6) | 5.81 (5.18) | 5.6 |
| 27b | 2,3-Biphosphoglycerate-independent phosphoglycerate mutase (At) | At3g08590 | Carbohydrate metabolism | 3 | 65.5 (60.7) | 5.81 (5.43) | 6.3 |
| 28 | GST (GST6; At) | At2g47730 | Redox processes | 3 | 28.3 (19.7) | 6.30 (5.22) | 26.0 |
| 29 | GST (Bj) | gi2204102 | Redox processes | 2 | 34.1 (15.4) | 6.40 (5.17) | 15.1 |
| GST (At) | At1g78340 | Redox processes | 2 | 34.1 (25.2) | 6.40 (4.99) | 5.5 | |
| 30 | Glyceraldehyde-3-P dehydrogenase (At) | At1g13440 | Carbohydrate metabolism | 10 | 41.5 (36.9) | 6.56 (6.79) | 27.2 |