Figure 5.

TGA2 is a negative regulator of PR gene expression. Northern blot showing expression of PR1 and PR2 in tga2-2 sni1-1 and tga2-2 sni1-1 npr1-1 mutants. Total RNA was extracted from 2-week-old seedling grown on Murashige and Skoog agar plates with (+) or without (−) INA (20 μm). PR1 and PR2 expression levels were analyzed by hybridizing an RNA blot with respective gene-specific probes. A UBQ5-specific probe was used to hybridize the same blot as a loading control. WT, Wild type; UBQ, ubiquitin.