Table I.
Summary of primer pairs used for genotyping and RT-PCR
Primer pairs listed in the first column were used to amplify either the genomic DNA or the cDNA from wild-type and mutant plants. The gene names are given before the kind of template used for amplification.
| Primer Pair | Amplification |
|---|---|
| TGAKOF + TGAKOR | TGA1 wild type genomic |
| LBb1 + TGA1KOF | TGA1 T-DNA insertion |
| KOTGA2 + TGA2RKO | TGA2 wild type genomic |
| KOTGA2 + JL202 | TGA2 T-DNA insertion |
| TGA4KOF + TGA4KOR | TGA4 wild type genomic |
| LBb1 + TGA4KOF | TGA4 T-DNA insertion |
| KOT6 + TGA6RKO | TGA6 wild type genomic |
| JL202 + TGA6RKO | TGA6 T-DNA insertion |
| TGA7F + TGA7LCR | TGA7 wild type genomic |
| TGA1LCF + TGA1LCR | TGA1 cDNA |
| TGA2LCF + TGA2LCR | TGA2 cDNA |
| TGA3LCF + TGA3LCR | TGA3 cDNA |
| TGA4LCF + TGA4LCR | TGA4 cDNA |
| TGA6LCF + TGA6LCR | TGA6 cDNA |
| TGA7LCF + TGA7LCR | TGA7 cDNA |