Fig. 4.

Effects of U0126 on CHOP expression in β-cells and islets. (A–C) Expression of CHOP protein in Min6 cells and human islets in 5.5 mM glucose or 25 mM glucose (A); Min6 cells in 5.5 mM glucose in the presence or absence of U0126 (B); INS-1 cells in 5.5 mM glucose in the presence or absence of U0126 or UK14304 (C). (D) Activation of ERK1/2 in INS-1 cells by 11 mM glucose in the presence or absence of UK14304. Blots of islet proteins were repeated twice and, for cell lines, a minimum of three times. The time course of D was repeated twice.