Fig. 5.

Effect of stresses on CHOP protein expression. (A) Min6 cells were maintained in 25 mM glucose then treated with or without LPS or hydrogen peroxide in the absence or presence of U0126. (B) Min6 cells in 25 mM or 5.5 mM glucose with or without thapsigargin (Thaps) or tunicamycin (Tunic) in the presence or absence of U0126. (C) Promoter–reporter assays of CHOP gene promoter −954 to +1 bp in Min6 maintained in 5.5 or 25 mM glucose then exposed to thapsigargin (Tg) or tunicamycin (Tu) for 4 h. Blots were repeated three times. Promoter assays were repeated a minimum of three times. U, U0126.