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. 2007 Jul 2;104(28):11643–11648. doi: 10.1073/pnas.0701877104

Fig. 3.

Fig. 3.

Analysis of TNF and IFN-γ-induced gene transcription in Piasy−/−Pias1+/− and Piasy+/+Pias1+/− MEFs. (A) Western blot analyses were performed with whole-cell lysates prepared from various cell types as indicated, by using Abs against PIAS1, PIASy, and tubulin. (B) Piasy+/+Pias1+/− and Piasy−/−Pias1+/− MEFs were untreated or treated with TNF (20 ng/ml) for various time points. Total RNA was prepared and subjected to Q-PCR analyses. (C) Same as in A except cells were treated with IFN-γ. (D) Piasy+/+Pias1+/− and Piasy−/−Pias1+/− MEFs were infected with MHV-68, followed by Western blot analyses with anti-ORF-26, anti-M9, or anti-tubulin.