Fig. 4.

ECE-1 regulation of receptor resensitization. (A) In KNRK-NK₁R cells, overexpression of ECE-1a–d accelerated resensitization at 5–60 min. (B) In HEK-NK₁R cells, SM-19712 inhibited resensitization at 120 min. (C) In HEK-NK₁R cells, SM-19712 did not affect the initial response to SP (10 nM). (D) In HEK-NK₃R cells, SM-19712 inhibited NKB resensitization at 30 min. (E) In mice, SP-induced extravasation of Evan's blue in the skin was desensitized when the interval between SP doses was 30 min, and resensitization was complete after 480 min. (F) In mice, SM-19712 inhibited resensitization at 60 min. ∗, P < 0.05; ∗∗, P < 0.01 to KNRK-NK₁R cells or vehicle.