Fig. 2.
Loss of telomerase activity in the ter1-ΔΔ strain. (Left) Extracts and DEAE fractions were prepared from the BWP17, ter1-ΔΔ, and ter1-ΔΔ/TER1 strains and subjected to primer extension assays using the indicated primer and 32P-labeled dTTP. As controls, fractions were also pretreated with RNase A before the assays. (Right) Telomerase fractions from the BWP17 and ter1-ΔΔ strains were tested in primer extension assays either separately or after mixing as indicated. A 46-nt labeled oligonucleotide was added to each reaction mixture before ethanol precipitation as a control for sample recovery (LC).