Figure 5.

Virulence in macrophages and SCID mice. THP-1 cells were infected with the wild-type and mutant strain at an MOI of (A) 1:50 and (B) 1:5 bacteria to macrophages. (■) Wild-type; (□) glnD mutant. Bacterial numbers (as determined by CFU counts) are the mean±standard deviation from triplicate wells monitored over 7 days. (C) Survival of SCID mice after infection with 10⁶ bacteria. Each group contained six mice. (□) Wild-type, (○) Tame 69, (◊) control (PBS).