Table 3.

Ectopic arg-1::gfp expression upon overexpression of CeTwist alone or with CeE/DA.

Strain name	heat shock plasmidsa	arg-1::gfp reporter	heat shock 20 minb		heat shock 2 hrb
			recover 2 hr	recover 20 hr	recover 2 hr	recover 20 hr
AK100c	phs::hlh-8	none	−d	−	−e	−
AK107	none	pZJ19 [−436 to −1]	−	−	−	−
AK105c	phs::hlh-8	pZJ19 [−436 to −1]	+/−	+	+	+++
AK104	phs::hlh-8 phs::hlh-2	pZJ19 [−436 to −1]	++	++++	++	++++

^aIntegrated plasmids containing cDNA under the control of the hsp16.41 promoter.

^bPopulations of mixed stage animals of each strain were subjected to heat shock treatment at 33°C for 20 min or 2 hrs followed by recovery at 20°C. The GFP expression pattern of the animals was examined under the fluorescent stereomicroscope during recovery.

^cStrains AK100 and AK105 also contain hlh-8::gfp, which is a transcriptional reporter that does not contain any coding sequence. This gfp construct was linked to the integrated phs::hlh-8 and could not be crossed out of the strain. However, the difference between the response to heat shock in AK100 compared to AK105 is specific to arg-1::gfp since AK100 has essentially no ectopic expression from hlh-8::gfp.

^d-:no ectopic GFP expression compared to animals without heat shock treatment; +/−: low level of ectopic GFP expression; +: modest level of ectopic GFP expression; each additional + indicates a significant increase in GFP expression.

^eSporadic, low level of ectopic hlh-8::gfp expression was observed in embryos but not larvae after heat shock treatment for 2 hrs and recovery for 4 hrs; the GFP expression disappeared after recovery for 8 hrs.