Skip to main content
. Author manuscript; available in PMC: 2007 Jul 11.
Published in final edited form as: Free Radic Biol Med. 2007 Jan 31;42(9):1398–1408. doi: 10.1016/j.freeradbiomed.2007.01.040

Figure 2.

Figure 2

Dicumarol blocks the NE-induced increase in MUC5AC mRNA expression in NHBE cells. NHBE cells grown on Transwell clear chambers (10 days at ALI) were starved in media supplemented with only two factors, BSA and RA, for 24h and treated with dicumarol (4 or 10 μM) or vehicle 1h prior to and then during NE (500 nM, 1h) or control vehicle treatment. Total RNA was collected by Trizol. MUC5AC mRNA expression was evaluated by quantitative real-time RT-PCR. The relative MUC5AC mRNA level was normalized to 18S rRNA and expressed as percent of control treated cells. Data are presented as mean ± SEM (3 separate experiments with total n of 4-6). The dashed line at 100% represents control levels in the absence of dicumarol and NE.*, significantly different from control treated cells (P=0.002). #, significantly different from NE alone (P = 0.041).