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. Author manuscript; available in PMC: 2007 Jul 11.
Published in final edited form as: Free Radic Biol Med. 2007 Jan 31;42(9):1398–1408. doi: 10.1016/j.freeradbiomed.2007.01.040

Figure 4.

Figure 4

NQO1-knockdown by siRNA transfection abrogates the NE-induced upregulation of MUC5AC mRNA expression. A549 cells (70% confluent) were transfected with siRNA for NQO1 (siNQO1, 20 nM), or control siRNA (siControl, 20 nM). 22h post-transfection, cells were treated with NE (100nM) or control vehicle for 6h and total RNA was collected by Trizol. MUC5AC mRNA expression was evaluated by quantitative real-time RT-PCR and normalized to 18s rRNA expression. The relative MUC5AC mRNA levels from NE-treated cells were expressed as a percentage of their corresponding control-treated cells. Data are presented as mean ± SEM (n=9, 3 separate experiments). The dashed line at 100% represents control levels. *, significantly different from siControl transfected cells (P<0.04).