Dicumarol inhibited NE-induced lipid peroxidation in A549 cells. A549 cells were preincubated with dicumarol (30μM, 1h) or 0.1M NaOH and then treated with NE (100nM) or control vehicle for 2h. Cell lysate was collected and evaluated for lipid peroxidation by HPLC. Results are expressed as percent of control. D, dicumarol; NE, neutrophil elastase; NE+D, neutrophil elastase plus dicumarol. Data are presented as mean ± SEM (n=10, 2 separate experiments). The dashed line at 100% represents control levels. *, significantly different from control, P<0.006. #, significantly different from NE (P=0.021).