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. Author manuscript; available in PMC: 2007 Jul 11.
Published in final edited form as: Free Radic Biol Med. 2007 Jan 31;42(9):1398–1408. doi: 10.1016/j.freeradbiomed.2007.01.040

Figure 8.

Figure 8

NE increased NQO1 protein and activity level in the cytosolic fraction, not in the membrane fraction. Cytosolic and membrane fractions of A549 cells treated with NE (100nM, 1h) or control vehicle (C) were evaluated for NQO1 protein level by western blot (A). E-cadherin was used as a positive control for the plasma membrane fraction (B). The graph summarizes the densitometry data of NQO1 protein level in the cytosol normalized to Ponceau S staining. Results are expressed as a percent of control, mean ±SEM (n=6, 4 separate experiments). *, significantly different from control (P= 0.01) (C). NQO1 activity in the cytosolic fraction was evaluated using a spectrophotometric assay measuring the reduction of DCPIP at 600 nm. Relative NQO1 activity was expressed as a percent of control, mean ±SEM (n=6, 4 separate experiments). *, significantly different from control (P = 0.002)(D).