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. 2007 Jul 13;3(7):e117. doi: 10.1371/journal.pgen.0030117

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© 2007 Le Bourgeois et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Km^R cassette excision mediated by XerS in E. coli in different genetic backgrounds is shown. The +/− signs indicated presence/absence of plasmid expressing the L. lactis xerS (pCL297) or the E. coli ftsK (pCL263) genes. When available the number of independent experiments (n) is indicated below each excision frequency mean value. Error bars correspond to the standard deviation (σⁿ⁻¹).

(B) Effect of the lactococcal XerS/dif _SL system on chromosome dimer resolution in E. coli as measured by growth competition assays is presented. E368 (XerS ⁺ dif _SL ⁺) was mixed with E367 (XerS ⁺ dif _SL ⁻, squares), E378 (XerS⁺ dif _SL ⁺ ftsK _C ⁻, triangles), or E375 (WT strain with XerS, circles) at a ratio of 1:1 and grown in serial culture for 60 generations. Values were calculated from two independent assays.