Figure 3. Cid and Ald Localize at Drosophila Kinetochores in Mitosis and Meiosis.

This colocalization shows the spatial relationship of the inner and outer kinetochore proteins, with Cid marking the inner kinetochore and Ald marking the outer.

(A) A maximum intensity stack projection of DAPI staining in two adjacent colchicine-treated w¹¹¹⁸ larval neuroblast cells arrested at metaphase. Due to overlapping chromosomes, we have presented single optical sections of the stack (insets). Each inset is from a different optical section, with the connected border showing the location in the overall image. Each inset shows localization in a pair of sister chromatids of DNA (blue), Cid (red), and Ald (green) localization. In each case, the inner kinetochore protein Cid localized closer to the DNA than the outer kinetochore protein Ald.

(B) A prometaphase oocyte nucleus from FM7/yw, showing DNA (blue) and the tubulin spindle (red). Inset: a 2× enlargement of the same nucleus, showing DNA (blue), Cid (red), and Ald (green). Note the extended Ald staining at the kinetochores.

(C) A prometaphase oocyte nucleus from FM7/yw, showing DNA (blue), tubulin (red), and Ald (green). Note that the kinetochores are round foci, although the spots for the four chromosomes (arrows) are well-separated from the DAPI staining for those chromosomes.