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. 2007 Jul 13;3(7):e113. doi: 10.1371/journal.pgen.0030113

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© 2007 Gilliland et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) A 60× image of a polo-GFP oocyte, apparently fixed in early GVBD. Note the germinal vesicle is still present (dotted outline), but with “ruffling” of the membrane, indicating the germinal vesicle is beginning to break down. Only GFP (grayscale) and DAPI (blue) are shown for clarity; Ald (Cy3) showed colocalization to filaments, and tubulin (Cy5) showed no detectable spindle formation around the oocyte nucleus. Short and relatively uniform Polo-GFP filaments are present throughout the cytoplasm, but not within the germinal vesicle. Those filaments within the dotted outline were in different focal planes than the membrane, and were clearly outside the germinal vesicle. When compared to most oocytes with filaments (compare to wild type in Figure 4C, and Polo-GFP in Figure 6B), the cytoplasmic filaments are much shorter (most well under 5 μm). (B) Individual filaments from a FM7/yw oocyte, apparently fixed during GVBD, stained with anti-Ald (green) and anti-BubR1 (red) antibodies. Note the very similar lengths of the filaments and the presence of BubR1 foci at the tips of the Ald filaments.