Skip to main content
. 2007 Jun 14;26(13):3098–3108. doi: 10.1038/sj.emboj.7601756

Figure 7.

Figure 7

Interdependent Sch9 and Hog1 recruitment to the osmostress-responsive GRE2 and CTT1 genes in vivo. (A) Sch9 association with the indicated GRE2 and CTT1 promoter and ORF regions. Yeast wild type (MAP85) or hog1Δ mutants (MAP90) expressing Sch9-(HA)3 or the no tag control strain W303-1A were used. ChIP was performed with unstressed cells (−NaCl) or after brief osmostress (5 min, 0.4 M NaCl). (B) Hog1 association with the same chromosomal regions. Hog1-HA was expressed in wild type (MAP51) or in sch9Δ mutants (MAP83), before and after osmotic shock. (C) Sch9 association with the GRE2 promoter and ORF region. Yeast wild type (MAP85) or sko1Δ mutants (MAP93) expressing Sch9-(HA)3 were used before or after brief hyperosmotic shock. (D) Kinetic ChIP analysis of Sch9-(HA)3 association at the GRE2 and CTT1 promoters. (E) A schematic representation of the amplified regions in the ChIP experiments. Immunoprecipitation (IP) efficiencies are presented as the fold over the POL1 coding sequence control. Expression of HA-tagged Sch9 and Hog1 was not affected in the mutant strains used, as detected by Western blot.