Figure 5.

Acetylation of p53, a SIRT1 substrate, in p25 transgenic mice reversed by resveratrol. (A) Upregulation of p53 in p25 transgenic mice (n=4) detected by immunoprecipitation followed by Western blot. Densitometry analyses of p53 levels are shown on right. (B) Acetylation of p53 at lysine 382 in p25 transgenic mice (n=3) detected by immunoprecipitation, followed by Western blot. ^* Indicates nonspecific band. (C) P53 knockdown in p25-expressing primary hippocampal neurons rescues p25 neurotoxicity by 25%. ^**P(T⩽t) two tails: 0.001. (D) Efficient knockdown of p53 by RNAi in cell line transfected with p53. (E) Reduced acetylation of p53 at lysine 382 and downregulation of p53 in p25 transgenic mice (n=3) treated with resveratrol. Densitometry analyses of acetylated p53 levels is shown in the bottom panel.