Figure 2.

The OE17 moiety of pOE17-GS15-protA is transported across the thylakoid membrane, whereas the protein A moiety remains on the stromal side of the thylakoid membrane. Thylakoids recovered from a transport assay (Materials and methods) with pOE17-GS15-protA were thermolysin treated (lane 3) or not (lane 2), as shown below the panel, and were subjected to denaturing immunoprecipitation (IP, lanes 4–7) with antibodies to OE17, or an irrelevant antibody, as described in Materials and methods and depicted below the panel. Translation product (lane 1), 2% of assay; thylakoids and thermolysin-treated thylakoids, 50% of assays; eluates from the antibody beads, 100% of assays.