Figure 5.
Transport of pOE17-GS-mOE17, pOE17-GS15-(mOE17)2, and pOE17-GS-protA into thylakoids. (A) pOE17-GS15-(mOE17)2 was incubated with washed thylakoids in a transport assay. Recovered thylakoids were either mock treated (mk) or treated with thermolysin, in the absence or presence of 1% Triton X-100, as shown below the panels. The gel in the right panel was exposed to film for three times as long as the left panel. (B) Concurrent assay of all precursors in parallel washed thylakoid transport assays. Recovered thylakoids were mock treated (mk) or treated with thermolysin, as shown. All panels were from the same gel, but exposed to film for different times, to adjust for different translation efficiencies. Numbers below the lanes represent the percentage of processed protein that was arrested mid transport. This was calculated from the DPM of the degradation product adjusted for the number of leucine residues in the mature protein versus degradation product. Translation products represent 6.5% and all other samples represent 100% of the assay mixtures.