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Separation of PSII monomers and dimers by ultracentrifugation through a glycerol density gradient. PSII corresponding to 5 μg Chl was purified from wild-type (WT) and pgsA mutant (−PG) cells grown in PG-free medium. Monomeric and dimeric forms of purified PSII were separated by ultracentrifugation through a 5% to 30% glycerol density gradient. An additional green band of unknown identity in PSII from mutant cells is indicated by an asterisk.
