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Effects of PG on rebinding of PsbO to PSII. Thylakoid membranes were isolated from wild-type (WT) and pgsA PG-depleted mutant cells (−PG), or from mutant cells that were reincubated with PG in the absence (+PG/−Lin) or presence (+PG/+Lin) of lincomycin for 10 h. Distribution of D1 and PsbO in the individual fractions from a 5% to 30% glycerol gradient was analyzed by western blotting using antibodies against each protein.
