Figure 1.

Means whereby processed nuclease protection probes are captured and detected at specific array elements in the ArrayPlate. The S1-processed lysates (Supplemental Fig. S1) are added to the corresponding wells of a plate previously programmed by addition of linkers to capture each specific nuclease protection probe. These nuclease protection probes hybridize to their complementary array-bound programming linker. Detection linkers are added next, each hybridizing to its respective nuclease protection probe. The media are then aspirated and replaced with media containing detection probes. Detection probes hybridize to each array-bound detection linker. The plate is then washed and substrate for HRP is added, producing chemiluminescence at each array element location, which is then quantitatively imaged.