Figure 5.

Dose-response of Arabidopsis transcripts to NaCl treatment. Arabidopsis plants, grown from seed for 10 d in 96-well plates, were treated with the indicated amounts of NaCl for 4 h and then harvested. A, Data are from quadruplicate biological samples and ses are shown by error bars. Data are normalized to the average of the transcript levels of actin and S-19. Note that two genes (KIN1 and KIN2) exhibit an EC₅₀ of approximately 112 mm, whereas a third, COR47, exhibits an EC₅₀ >175 mm. B, Scale of the ordinate is expanded to illustrate the sensitivity of the assay without loss of reproducibility.