Table I.
RI50 values and mutations in AtGPRAT2 nucleotide and polypeptide sequences for seven Arabidopsis mutant lines resistant to DAS734
The asterisks separate three groups with high (***, >110-fold), moderate (**, approximately 58-fold), and low (*, approximately 5-fold) levels of resistance.
| Line | RI50a | Fold Resistance | Changes in AtGPRAT2 Sequence
|
|
|---|---|---|---|---|
| Nucleotideb | Amino Acid | |||
| μm | ||||
| Wild type | 0.2 | – | – | – |
| R03*** | 20 | 111 | G791A | R264K |
| R06*** | 35 | 145 | G791A | R264K |
| R01** | 14 | 58 | C1426T, A388G | P476S, T130A |
| R07** | 14 | 58 | C1426T, A416Gc | P476S |
| R08** | 10 | 56 | C1426T, C1246Tc | P476S |
| R04* | 1.0 | 6 | C793T, A1482T | P265S, Y494F |
| R22* | 0.9 | 5 | G1110A | G371S |
a
Determined from dose responses of seedlings grown in agarose media.
b
Numbered from the initiator TAG codon.
c
Silent mutation.