Figure 2.

NIP1-elicited PR5 mRNA accumulation and H⁺-ATPase stimulation. A, RNA gel blot using RNA from primary leaves of barley ‘Atlas 46’ (Rrs1) and ‘Atlas’ (rrs1) 24 h posttreatment with NIP1. Leaves were either inoculated with spores of the NIP1 type I-expressing fungal isolate UK7 (F) or treated with 10 μL of a NIP1 solution containing 25 μm NIP1 type I, II, III*, IV*, respectively, in 0.05% Tween 20. As a control (C), leaves were treated with 10 μL of 0.05% Tween 20. B, RNA gel blot using RNA from primary leaves of barley ‘Atlas 46’ 24 h posttreatment with 10 μL of NIP1 type I (I) or type II (II) solutions containing increasing protein concentrations (1, 0.77 μm; 2, 1.55 μm; 3, 2.34 μm). Nonlinear regression analysis (C) of a representative RNA gel blot after phosphoimager-assisted quantification and of H⁺-ATPase stimulation (D; mean values from Wevelsiep et al., 1991, 1993). Type I, ▪; type II, ▴.