Figure 1.

Suc density gradient profiles of thylakoids from dark-adapted sun and shade leaves of I. sapindoides. Some of the shade leaves were exposed to approximately 200 μmol m⁻² s⁻¹ for 30 min prior to the dark adaptation (shadeL). Distribution profiles of Chl a (A) and Chl b (B) among the seven fractions obtained (bands 1–7, from the lowest to the highest density) are shown. Concentrations of the pigments are given relative to the total (=100%). Polypeptide compositions of the seven bands were then analyzed by denaturing SDS-PAGE (C) and identified by western blotting using specific antibodies (data not shown). Solubilized thylakoids of I. sapindoides were also separated by nondenaturing Deriphat-PAGE and compared with thylakoids from Arabidopsis under the same condition (D).