Pigment composition of the recombinant Lhcb1 and Lhcb5
Recombinant Lhcb1 (420 μg of apoprotein) was reconstituted with 25 μg N, 65 μg L, Lx, or L:Lx (1:1), and 240 μg Chls (Chl a/b = 2.3). The same amount of recombinant Lhcb5 was reconstituted with 60 μg L, Lx, or L:Lx (1:1), and 240 μg Chls (Chl a/b = 3.0). The Lx pigment used for the reconstitution contained approximately 10% A. Samples were then separated from free pigments by Suc density gradient. Xanthophyll concentrations are normalized to 12 or nine Chl molecules for Lhcb1 and Lhcb5, respectively, according to the previous studies (see text). sd < ±0.1.