Table II.

Analysis of confirmed mutant alleles

Mutant Allele	Confirmed Insertion Sitea	Siliques Screened	Seeds Screened	Aborted Seedsb	Top Halfc	Average Seed Size	Terminal Phenotype
				%		μm
hisn2-1	Exon 5 (5)	25	898	16.0***	71.5***	320	Preglobular
		50	1,997	20.8***	62.9***		Preglobular
hisn2-2	Exon 5 (5)	50	1,672	16.0***	69.3***	220	Preglobular
		60	2,346	19.9***	65.2***		Preglobular
hisn2-3	Promoter	50	2,117	23.9	55.9**	340	Preglobulard
hisn3-1	Exon 1 (7)	50	2,168	21.7***	72.9***	270	Preglobular
		60	2,201	22.0**	66.8***		Preglobular
hisn3-2	Exon 5 (7)	50	2,092	20.7***	72.3***	280	Preglobular
hisn4-1	Exon 1 (19)	50	2,059	21.8**	64.7***	380	Preglobular
		45	1,830	20.0***	65.0***		Preglobular
hisn4-2	Intron 14 (18)	50	2,114	23.4	65.8***	330	Preglobular
		50	1,926	20.9***	64.5***		Preglobular
hisn4-3	Intron 17 (18)	50	2,161	23.5	58.7***	330	Preglobular
hisn6a-1	Exon 2 (8)	50	1,787	28.7***	53.2	440	Preglobulare
		25	1,053	25.0	54.4	340	Preglobular
hisn6a-2	Exon 7 (8)	25	1,228	25.4	59.2***	370	Preglobular
		10	396	23.4	55.6		Preglobular
hisn8	Exon 10 (11)	110	4,756	[24.5]f	N.D.	<100	Aborted ovule

^aBased on alignment of flanking sequence; total number of exons or introns noted in parentheses.

^bPresent in heterozygous siliques; 25% expected; significant deviation: **, P < 0.01; ***, P < 0.001.

^cAborted (homozygous mutant) seeds in top half of heterozygous siliques; 50% expected. Significant deviation indicates probable effect of mutation on pollen tube growth. N.D., Not determined.

^dOccasional seeds with cotyledon defects of unknown origin also found in this line and included in totals.

^eOccasional seeds with delayed phenotype attributed to heterozygotes included in totals for this population.

^fPercentage of aborted ovules and early aborted seeds combined.