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Plasmalemma localization of the AhHMA4:GFP fusion protein in tobacco plants, viewed by confocal laser scanning microscopy. Transient expression of AhHMA4:GFP in tobacco leaf epidermal cells. A, Bright-field image of the tobacco leaf epidermis. The nucleus is indicated with an arrow. B, GFP fluorescence in the same cell concentrated to the plasmalemma, which followed the cell contour, outside the nucleus. C, Pseudocolored merged image with GFP in green. Bar = 40 μm.
