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Cd content in BY4741 expressing AhHMA4, AhHMA4D401G, or AhHMA4-C. Yeast BY4741 cells transformed with the pYES2-GFP plasmid and with the pYES2-GFP containing AhHMA4, AhHMA4G401D, or AhHMA4-C were grown in liquid SD induction media with (hatched bars) or without 20 μm CdSO4 for 48 h (white bars). Metal contents were analyzed by atomic absorption spectroscopy. Results are an average (±se) from three independent experiments.
